Purification and partial characterization of an alkaline lipase from Pseudomonas pseudoalcaligenes F-111

Purification and partial characterization of an alkaline lipase from Pseudomonas pseudoalcaligenes F-111.

An extracellular alkaline lipase of alkalophilic Pseudomonas pseudoalcaligenes F-111 was purified to homogeneity. The apparent molecular weight determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was 32,000, and the isoelectric point was 7.3. With p-nitrophenyl esters as its substrates, the enzyme shows preference for C12 acyl and C14 acyl groups. It was stable in the pH ran...

Purification and characterization of nitrobenzene nitroreductase from Pseudomonas pseudoalcaligenes JS45.

Pseudomonas pseudoalcaligenes JS45 grows on nitrobenzene as a sole source of carbon, nitrogen, and energy. The catabolic pathway involves reduction to hydroxylaminobenzene followed by rearrangement to o-amino-phenol and ring fission (S. F. Nishino and J. C. Spain, Appl. Environ. Microbiol. 59:2520, 1993). A nitrobenzene-inducible, oxygen-insensitive nitroreductase was purified from extracts of ...

Production, Partial Purification and Characterization of an Extracellular Psychrotrophic Lipase from Pseudomonas Sp. ADT3

Psychrotrophic Pseudomonas ADT3 (NCBI GenBank Acc.no.JX914667) is capable of growth on lipid as the sole carbon source. In this paper, we report the purification and characterization of an extracellular lipase from psychrotroph, isolated from soil sample of NyAlesund, Svalbard, Arctic region. The Pseudomonas ADT3 isolate produces lipase enzyme in the extracellular minimal media with only 1% oli...

PURIFICATION AND PARTIAL CHARACTERIZATION O F PEROXIDASES FROM CULTIVATED RAPHANUS SATIVUS L. VAR. CICIL

Two peroxidases (EC. 1.11.1.7), POD I and POD II were purified from the roots of cultivatedRaphanus sativus L. Var. Cicil by one step ion-exchange chromatography after fractionation by acetone. The molecular weight of these enzymes were 43000 and 41000 Daltons and RZ 1.2 and 2.0 for POD I and POD II, respectively. Both enzymes consisted of a single polypeptide chain on SDS-PAGE. The maximum...

PARTIAL PURIFICATION AND CHARACTERIZATION OF LIPASE ENZYME FROM A Pseudomonas STRAIN